Sperm Morphology of Domestic Animals. Jennifer Koziol
Table of Contents
1 Cover
5 Preface
7 Introduction I.1 Spermatogenesis I.2 Length of Spermatogenesis in Domestic Species I.3 Maturation of Spermatozoa in the Epididymis I.4 Staining Techniques and Evaluation of Morphology I.5 Vital Staining I.6 Preparation of Semen Smears or Coverslip Slides I.7 Differential Counting of Sperm Morphology
8 Section I: Head Abnormalities 1 Pyriform and Tapered Heads 2 Nuclear Vacuolation, Including the Diadem Defect 3 Macrocephalic and Microcephalic Sperm 4 Rolled Head–Nuclear Crest–Giant Head Syndrome 5 Abnormal DNA Condensation 6 Acrosome Abnormalities 7 Normal Detached Heads and Free Abnormal Heads 8 Decapitated Sperm Defect
9 Section II: Midpiece Abnormalities 9 Proximal Cytoplasmic Droplet 10 Pseudodroplet 11 Mitochondrial Sheath Defects 12 Corkscrew Sperm Defect 13 Dag Defect 14 Distal Midpiece Reflex 15 Bowed Midpieces 16 Distal Droplets 17 Abaxial Midpieces
10 Section III: Tail (Principal Piece) Abnormalities 18 Tail Stump Defect 19 Coiled Principal Pieces 20 Double Forms and Accessory Tails 21 Bent Principal Pieces 22 Short Tail Defect
11 Section IV: Aberrations of Stains and Other Cells in the Ejaculate 23 Aberrations Due to Staining 24 Aberrations Due to Cold Shock 25 Round Cells in the Ejaculate 26 Teratoids 27 Medussa “Cells” 28 Aberrations of Semen Quality 28.1 Oligospermia/Azoospermia 28.2 Hemospermia 28.3 Urospermia 28.4 Antisperm Antibodies
12 References
13 Index
List of Tables
1 IntroductionTable I.1 Mean time of sperm epididymal passage in domestic
List of Illustrations
1 IntroductionFigure I.1 Morphologically normal sperm from a bull (eosin–nigrosin, 1000×)....Figure I.2 Morphologically normal sperm from a bull (phase contrast wet moun...Figure I.3 Morphologically normal sperm from a buck (eosin–nigrosin, 1000×)....Figure I.4 Morphologically normal sperm from a stallion (eosin–nigrosin, 100...Figure I.5 Partially stained sperm with a pink posterior and a white anterio...Figure I.6 Poorly prepared slide with too many sperm per field, which impede...Figure I.7 A small line of stain is placed along the slide followed by a sma...Figure I.8 Using a second slide, one can start in the stain backing up to pi...Figure I.9 Slides should neither be too dark or too light, and when viewed u...
2 Chapter 1Figure 1.1 Pyriform head in a bull (eosin–nigrosin, 1000×).Figure 1.2 Pyriform head in a bull as indicated by arrow (eosin–nigrosin, 10...Figure 1.3 Pyriform head in a bull as indicated by arrow (eosin–nigrosin, 10...Figure 1.4 Detached pyriform head in a bull indicated by arrow (eosin–nigros...Figure 1.5 Pyriform head in bull as indicated by arrow (eosin–nigrosin, 1000...Figure 1.6 Pyriform head in a bull as indicated by arrow (eosin–nigrosin, 10...Figure 1.7 Pyriform head in bull as indicated by arrow also note the proxima...Figure 1.8 Pyriform head in a bull (eosin–nigrosin, 1000×).Figure 1.9 Pyriform head in a ram (eosin–nigrosin, 1000×).Figure 1.10 Pyriform head in a bull (eosin–nigrosin, 1000×).Figure 1.11 Pyriform head defect in a bull as indicated by arrow compared to...Figure 1.12 Pyriform‐shaped head in a bull with proximal droplet (phase cont...
3 Chapter