Understanding Platelet-Rich Fibrin. Richard J. Miron
mean ± SE; an asterisk (*) indicates a value significantly higher than the control group (P < .05); a double asterisk (**) indicates a value significantly higher than all other groups (P < .05); a number sign (#) indicates a value significantly lower than all groups (P < .05). (Reprinted with permission from Fujioka-Kobayashi et al.11)
Conclusion
In summary, the results from the 24-protocol investigation demonstrated clearly that certain protocols were better at increasing the amount of platelet/leukocyte yield (400g to 700g for 8 minutes), whereas others were more effective at concentrating PRF (200g to 300g for 5 minutes). Therefore, new protocols were designed accordingly for solid-PRF and liquid-PRF. While histologically it was observed that horizontal centrifugation led to better cell layer separation of blood cells as well as more even distribution of cells throughout the PRF clot, it was also histologically observed that the majority of cells accumulated on the back distal surfaces of PRF tubes when fixed-angle centrifugation was carried out. Lastly, a new C-PRF formulation was evaluated demonstrating up to a threefold increase in GF release during a 10-day period and further elicited fourfold increases in gingival fibroblast migration, PDGF gene expression, and collagen1 synthesis when compared to standard i-PRF protocols.
References
1.Miron RJ, Chai J, Zheng S, Feng M, Sculean A, Zhang Y. A novel method for evaluating and quantifying cell types in platelet rich fibrin and an introduction to horizontal centrifugation. J Biomed Mater Res A 2019;107:2257–2271.
2.Miron RJ, Dham A, Dham U, Zhang Y, Pikos MA, Sculean A. The effect of age, gender, and time between blood draw and start of centrifugation on the size outcomes of platelet-rich fibrin (PRF) membranes. Clin Oral Investig 2019;23:2179–2185.
3.Takahashi A, Tsujino T, Yamaguchi S, et al. Distribution of platelets, transforming growth factor-beta1, platelet-derived growth factor-BB, vascular endothelial growth factor and matrix metalloprotease-9 in advanced platelet-rich fibrin and concentrated growth factor matrices. J Investig Clin Dent 2019;10:e12458.
4.Tsujino T, Masuki H, Nakamura M, et al. Striking differences in platelet distribution between advanced-platelet-rich fibrin and concentrated growth factors: Effects of silica-containing plastic tubes. J Funct Biomater 2019;10:43.
5.Fujioka-Kobayashi M, Kono M, Katagiri H, et al. Histological comparison of platelet rich fibrin clots prepared by fixed-angle versus horizontal centrifugation [epub ahead of print 18 April 2020]. Platelets doi: 10.1080/09537104.2020.1754382.
6.Zhang J, Yin C, Zhao Q, et al. Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells. J Biomed Mater Res A 2020;108:61–68.
7.Ghanaati S, Booms P, Orlowska A, et al. Advanced platelet-rich fibrin: A new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 2014;40:679–689.
8.Miron RJ, Chai J, Fujioka-Kobayashi M, Sculean A, Zhang Y. Evaluation of 24 protocols for the production of platelet-rich fibrin. BMC Oral Health (in press).
9.Miron RJ, Fujioka-Kobayashi M, Hernandez M, et al. Injectable platelet rich fibrin (i-PRF): Opportunities in regenerative dentistry? Clin Oral Investig 2017;21:2619–2627.
10.Varela HA, Souza JCM, Nascimento RM, et al. Injectable platelet rich fibrin: Cell content, morphological, and protein characterization. Clin Oral Investig 2019;23:1309–1318.
11.Fujioka-Kobayashi M, Katagiri H, Kono M, et al. Improved growth factor delivery and cellular activity using concentrated platelet-rich fibrin (C-PRF) when compared with traditional injectable (i-PRF) protocols [epub ahead of print 7 May 2020]. Clin Oral Investig doi: 10.1007/s00784-020-03303-7.
12.Miron RJ, Chai J, Zhang P, et al. A novel method for harvesting concentrated platelet-rich fibrin (C-PRF) with a 10-fold increase in platelet and leukocyte yields. Clin Oral Investig 2020;24:2819–2828.
Конец ознакомительного фрагмента.
Текст предоставлен ООО «ЛитРес».
Прочитайте эту книгу целиком, купив полную легальную версию на ЛитРес.
Безопасно оплатить книгу можно банковской картой Visa, MasterCard, Maestro, со счета мобильного телефона, с платежного терминала, в салоне МТС или Связной, через PayPal, WebMoney, Яндекс.Деньги, QIWI Кошелек, бонусными картами или другим удобным Вам способом.