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channels produced in the vicinity of the cell membrane can lead to the electrical excitation of the nerve axon. The excitation arises from the effect of the membrane potential on the movement of ions, and from interactions of the membrane potential with the opening and closing of voltage activated membrane channels. The membrane potential increases when the membrane is polarized with a net negative charge lining the inner surface and an equal but opposite net positive charge on the outer surface. This potential may be simply related to the amount of electrical charge Q, using:

      where Q is in terms of Coulombs cm−2, Cm is the measure of the capacity of the membrane and has units farads cm−2 and E has units of volts. In practise, in order to model the APs the amount of charge Q+ on the inner surface (and Q on the outer surface) of the cell membrane has to be mathematically related to the stimulating current I stim flowing into the cell through the stimulating electrodes. Figure 3.1b illustrates how the neuron excitation results in generation of APs by acting as a signal converter [11].

      Hodgkin and Huxley estimated the activation and inactivation functions for the Na and K currents and derived a mathematical model to describe an AP similar to that of a giant squid. The model is a neuron model that uses voltage‐gated channels. The space‐clamped version of the Hodgkin–Huxley model may be well described using four ordinary differential equations [12]. This model describes the change in the membrane potential (E) with respect to time and is described in [13]. The overall membrane current is the sum of capacity current and ionic current as:

      (3.14)equation

Schematic illustration of the Hodgkin–Huxley excitation model.

      (3.15)equation

      INa can be related to the maximal conductance images, activation variable aNa , inactivation variable hNa , and a driving force (E – ENa ) through:

      (3.16)equation

      Similarly Ik can be related to the maximal conductance images, activation variable aK , and a driving force (E – EK ) as:

      (3.17)equation

      and Ileak is related to the maximal conductance images and a driving force (E – El ) as:

      (3.18)equation

      The changes in the variables aNa , ak , and hNa vary from 0 to 1 according to the following equations:

      (3.19)equation

      (3.20)equation

      (3.21)equation

      where α(E) and β(E) are respectively forward and backward rate functions and λt is a temperature‐dependent factor. The forward and backward parameters depend on voltage and were empirically estimated by Hodgkin and Huxley as:

      (3.22)equation

      (3.23)equation

      (3.24)equation

      (3.25)equation

      (3.26)equation

      (3.27)equation


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