Understanding Platelet-Rich Fibrin. Richard J. Miron

Understanding Platelet-Rich Fibrin - Richard J. Miron


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as a very short and slow centrifugation protocol of 700 rpm (60g) for 3 to 4 minutes. Following this protocol, i-PRF remained liquid for roughly 15 to 20 minutes. This new formulation was utilized for a variety of procedures including mixing with bone grafts to form a stable fibrin graft with improved handling and graft stability.

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      Quantifying Cell Types in PRF

      Pitfalls of current methods

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      A novel method to quantify PRF

      Over the past few years, several commercially available centrifuges have further been brought to market. These vary in many factors including protocols, RCF values, tube-rotor angulation, rotor radius size, and tube composition. Each of these plays a role in the final obtained PRF membrane, yet little data is scientifically available displaying cell numbers and content in the various layers following centrifugation.

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      By utilizing this novel technique and investigating each 1 mL layer by layer, it was possible for the first time to investigate exactly each cell layer following centrifugation and determine the precise location of each blood cell type.

      L-PRF protocol


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