The Fundamentals of Bacteriology. Charles Bradfield Morrey
appears in the protoplasm which seems to act somewhat like the centrosome of higher cells as a “center of attraction” so that the protoplasm gradually collects around it. The spot disappears or is enclosed in the collected protoplasm. This has evidently become denser as it is more highly refractive than before. In time all or nearly all of the protoplasm is collected. A new cell wall is developed around it which is thicker than the cell wall of the bacterium. This thickened cell wall is called the “spore capsule.” Gradually the remnants of the former cell contents and the old cell wall disappear or dissolve and the spore becomes “free” (Fig. 25).
Fig. 25.—The smaller oval bodies in the middle of the field are free spores.
If the spore is placed in favorable conditions the protoplasm absorbs water, swells, the capsule bursts at some point, a cell wall is formed and the bacterium grows to normal size and divides, that is, it is an active growing cell again. This process is called “germination” of the spore. The point at which the spore capsule bursts to permit the new cell to emerge is characteristic for each kind of bacterium. It may be at the end when the germination is said to be polar (Fig. 26). It may be from the middle of one side which gives equatorial germination (Fig. 27). Rarely it is diagonally from a point between the equator and the pole, which type may be styled oblique germination. In one or two instances the entire spore swells up, lengthens and becomes a rod without any special germination unless this type might be designated bi-polar.
Fig. 26.—Spores showing polar germination. The lighter part of the two organisms just below A and B is the developing bacterium. In the original slide the spore was stained red and the developing bacterium a faint blue.
Fig. 27.—A spore showing equatorial germination. The spore in the center of the field shows a rod growing out of it laterally. In the original slide the spore was stained red and the developing bacterium blue.
Fig. 28.—Spores in the middle of the rod without enlargement of the rod. The lighter areas in the rods are spores.
Fig. 29.—Spores in the middle of the rod with enlargement of the rod around them. The lighter areas in the rods are spores.
Spores are most commonly oval or elliptical in shape, though sometimes spherical. A spore may be formed in the middle of the organism without (Fig. 28) or with (Fig. 29) a change in size of the cell around it. If the diameter through the cell is increased, then the cell with the contained spore becomes spindle-shaped. Such a cell is termed a “clostridium.” Sometimes the spore develops in the end of the cell either without (Fig. 30) or with enlarging it (Fig. 31). In a few forms the spore is placed at the end of the rod and shows a marked enlargement. This is spoken of as the “plectridium” or more commonly the “drumstick spore” (Fig. 32). The position and shape of the spore are constant for each kind of bacteria. In one or two instances only, two spores have been observed in a single organism.
Fig. 30.—Spores in the end of the rod with no enlargement of the rod around them. The lighter areas in the rods are spores.
Fig. 31.—Spores in the end of the rod with enlargement of the rod, A, A, A, A.
Fig. 32.—Drumstick spores at the end of the rod.
The fact that the protoplasm is denser and the spore capsule thicker (the percentage of water in each is decidedly less than in the growing cell) gives the spore the property of much greater resistance to all destructive agencies than the active bacterium has. For example, all actively growing cells are destroyed by boiling in a very few minutes, while some spores require several hours’ boiling. The same relation holds with regard to drying, the action of chemicals, light, etc. That the coagulation temperature of a protein varies inversely with the amount of water, it contains, is shown by the following table from Frost and McCampbell, “General Bacteriology”:
| Egg albumin | plus | 50 per cent. water | coagulates at | 56° |
| Egg albumin | plus | 25 per cent. water | coagulates at | 74–80° |
| Egg albumin | plus | 18 per cent. water | coagulates at | 88–90° |
| Egg albumin | plus | 6 per cent. water | coagulates at | 145° |
| Egg albumin | dry | water | coagulates at | 160–170° |
This resistance explains why it happens that food materials boiled and sealed in cans to prevent the entrance of organisms sometimes spoil. The spores have not been killed by the boiling. It explains also in part the persistence of some diseases like anthrax and black leg in pastures for years. From the above description it follows that the spore is to be considered as a condensation of the bacterial protoplasm surrounded by an especially thick cell wall. Its function is the preservation of the organism under adverse conditions. It corresponds most closely to the encystment of certain protozoa—the ameba for example. Possibly the spore represents a very rudimentary beginning of a reproductive function such as is gradually evolved in the higher thread bacteria, the fission yeasts, the yeasts, the molds, etc. Its characteristics are so markedly different, however, that the function of preservation is certainly the main one.
It must not be supposed that spores are formed under adverse conditions only, because bacteria showing vigorous growth frequently form spores rapidly. Special conditions are necessary for their formation just as they are for the growth and other functions of bacteria (Chapters VI and VII).
CHAPTER III.
CELL FORMS.
Though there is apparently a wide variation in the shapes of different bacterial cells, these may all be reduced to three typical cell forms. These are: first and simplest, the round or spherical, typified by a ball and called the coccus form, or coccus, plural cocci4 (Fig. 33). The coccus may be large, that is, from 1.5µ to 2µ in diameter. The term macrococcus is sometimes applied to these large cocci. If the coccus is less than 1µ in diameter, it is sometimes spoken of as a micrococcus; in fact, this term is very commonly applied to any coccus. When cocci are growing together, many of the cells do not appear as true spheres but are more or less distorted from pressure of their neighbors or from failure to grow to full size after recent division. Most cocci divide into hemispheres and then each half grows to full size. A few cocci elongate before division and then appear oval or elliptical.
The second cell form is that of a cylinder or rod typified by a section of a lead-pencil. The name bacillus, plural bacilli, is applied to this type (Fig. 34). The bacillus may be short (Fig. 35), 1µ or less in length, or long, up to 40µ in rare cases. Most bacilli are from 2µ to 5µ or 6µ long. The ends of the rod are usually rounded, occasionally square and very rarely pointed. It is evident that a very short rod with rounded ends approaches a coccus in form and it is not always easy to differentiate in